DNA Extraction
We provide a rapid and affordable DNA extraction service which generates high quality, high yield DNA. Using Nucleon DNA extraction chemistry and other validated procedures (such as Qiagen, PAXgene, MagMax, OMEGA extraction kits) we are able to extract DNA from a broad range of biological matrices including:
- Nucleon Chemistry
- RNA/miRNA Extraction
Nucleon Chemistry (High Volume/High Yield for Bio-Banking)
The Nucleon extraction chemistry rapidly and efficiently extracts high yields of pure, high molecular weight DNA. Nucleon is compatible for use with whole blood and other sample types such as tissue and saliva. The DNA is not bound to a solid surface at any point in the process. This avoids exposing the DNA to shearing forces and promotes the recovery of high yields of highly intact DNA.
High yield (370-800μg/10mL blood)
High purity (A260/A280 > 1.8)
High MWt DNA (>50kb)
Economical large volume sample extraction
Fully scalable (0.3mL to 50mL blood and 5mg to 500mg tissue)
RNA/miRNA Extraction
We have proven experience in RNA/miRNA isolation from a number of different sample types using validated procedures such as TriZol, Qiagen, PAXgene, MagMax, ReliaPrep, Roche, OMEGA extraction kits.
Sample Types Include:
Formalin Fixed Paraffin Embedded Tissue (FFPE)
Whole blood (including samples collected into PAXgene/Tempus tubes)
Leukocytes collected into LeukoLOCKâ„¢ tubes
Plasma and sera
Saliva
Frozen tissue and biopsy material
Cultured cells (mammalian and bacterial)
