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Yourgene Health | LightBench® with Ranger® Technology

The LightBench® is a 3-in-1 automated size selection instrument. Powered by Ranger® Technology, the LightBench® is an ideal addition to any research laboratory.

LightBench® with Ranger® Technology

The LightBench®, powered by Ranger® Technology, is Yourgene’s research-grade size selection instrument. This versatile automated gel electrophoresis instrument offers multiple types of functionality. The gel electrophoresis is automatically monitored in real time for applications that require size selection, or simply for fragment length analysis. The instrument’s patented imaging system can also be leveraged for other fluorescence-based assays for quantification applications.

The accurate targeting of DNA fragment lengths for recovery through size selection enables more of the DNA of interest to be collected, helping to you to reduce sequencing costs which is ideal in a research laboratory environment. The LightBench® can be adapted easily for specific research workflows. Once you know the base pair range of interest, the agarose gel cassettes composition can be customised with chosen markers, to target specific base pair sizes.

Yourgene Health | LightBench® with Ranger® Technology
LightBench® with Ranger® Technology

LightBench®

The design of the LightBench® means it is easily scalable to meet increasing throughput requirements and can be integrated with existing automation platforms. We offer a wide range of complimentary reagents and kits. Please contact our customer service team to find out more.

Size Selection Applications

Parameter
Specification
Additional Information
Size Selection Applications
Size Selection Applications
Size Selection Applications
Allowable Size Range
0 - 20 kbp+
Different gel percentages allow size-selection targets across different regions of this total range
Loading Capacity
≤ 1 kb: 500 ng – 2 μg
2 - 5 kb: 250 ng – 1.5 μg
10 kb: 500 ng – 1 μg
20 kb+: 250 – 500 ng
Exact loading capacity depends on the size range of interest and the gel percentage chosen
Resolution
10 – 20%*
Depends on the reagent kit and the run conditions. Resolution is defined as the minimum difference in fragment length between the start or end of a size selection window and the peak of the closest off-target fragment that ensures at least 90% rejection of said off-target fragment
No. of Samples / Run
12 (CGI-Format)
8 (SBS-Format)
Depends on reagent kit selection
Run Time
1 – 4 hrs
Depends on optimisation of reagent kit selection and required resolution
Intrinsic Recovery Efficiency
70% - 80%
Dependent on run conditions, particularly the relationship between the target range and the extraction volume

*Resolution assumes the use of a size-selection cassette with 30 mm channel length. Size-selection cassettes with shorter channel lengths will have correspondingly lower resolution.

Electropherogram Trace Generation

Parameter
Specification
Additional Information
Acceptable Size Range
50 bp - 20 kbp+
Different gel percentages permit analysis across different regions of this total range
Sizing Accuracy
5-20% of nominal size
Depends on the fragment size and the reagent kit selection. When working within the recommended size-range for each gel type, sizing accuracy is typically within 5%
Lower Detection Limit
0.5 ng for constructs
5 ng for smears
Depends on the reagent kit and the run conditions. Resolution is defined as the minimum difference in fragment length between the start or end of a size selection window and the peak of the closest off-target fragment that ensures at least 90% rejection of said off-target fragment
No. of Samples / Run
24 - 48
This is a reagent kit parameter
Run Time
Typically 20 – 40 minutes
Depends on fragment size and reagent kit selection. Low voltage analysis of larger fragment (>20kb) can take significantly longer depending on the settings
Intrinsic Recovery Efficiency
70% - 80%
Dependent on run conditions, particularly the relationship between the target range and the extraction volume
Mass Calculation Accuracy
With in-gel calculation (no plate-based quant): + 40% With plate-based quant: + 20%**
The accuracy of the plate-based quant is primarily dependent on pipetting accuracy during sample-plate setup

*Resolution assumes the use of a size-selection cassette with 30 mm channel length. Size-selection cassettes with shorter channel lengths will have correspondingly lower resolution.

** with CG-14000 product series

Plate-Based Quantification

Parameter
Specification
Additional Information
Lower Detection Limit
0.2 ng
When using the prescribed Pico-Green-based assay. Other fluorophores may provide increased sensitivity
Accuracy
+/- 15%
Depending on pipetting accuracy and the use of replicates, this number can be brought down

*Resolution assumes the use of a size-selection cassette with 30 mm channel length. Size-selection cassettes with shorter channel lengths will have correspondingly lower resolution.

General

Parameter
Specification
Additional Information
Weight
20.5 kg
Dimensions (W x D x H)
32.5 cm x 42.4 cm x 38.1 cm

*Resolution assumes the use of a size-selection cassette with 30 mm channel length. Size-selection cassettes with shorter channel lengths will have correspondingly lower resolution.

Yourgene Health | the IONA® test

A Ranger® Technology Applications: Size Selection for NIPT, Oncology and Gene Synthesis Applications

In this edition of YourExpert, Yourgene Health interviewed Matthew Nesbitt, one of the developers of Ranger® Technology to discuss how its unique size selection function is helping our collaborators in life sciences and synthetic biology, across various clinical and research applications.

YEX014 Ranger® Technology Applications: Size Selection for NIPT, Oncology and Gene Synthesis Applications
YEX013 A Ranger® Technology Application Synthetic Biology and Gene Synthesis

A Ranger® Technology Application: Synthetic Biology and Gene Synthesis

In this edition of Your Expert, Yourgene Health interviewed Matthew Nesbitt, one of the developers of our breakthrough Ranger® Technology to discuss how its size selection function is revolutionising the approach to Gene Synthesis and how dozens of organisations are already benefitting from this function in the manufacture of their deliverables.

LightBench® enabling liquid biopsies for cancer detection

A Yourgene Health LightBench® customer, Dr Hunter Underhill is an Assistant Professor of Paediatrics within the medical genetics’ division at the University of Utah School of Medicine. He has a research lab at the Huntsman Cancer Institute studying the applicability of cfDNA detection for brain tumour surveillance and monitoring. Matthew Nesbitt from Yourgene interviewed Dr Underhill to better understand how his lab is seeking rare signals amongst noise using liquid biopsies for cancer detection.

Yourgene Health | Our Company | Your Expert | YEX004 LightBench - enabling liquid biopsies for cancer detection

Videos

Introducing Ranger® Technology

Size Selection of Cell-free DNA Increases the Proportion of Tumor Specific Variants in Cancer Patients.

Click here to view transcript

Welcome everyone to this on-demand webinar bought to you by Yourgene Health in collaboration with Labcorp. Today you will be hearing about how automated size selection using Ranger® Technology can help you get to the DNA you care about in liquid biopsy, and how Yourgene health’s solutions are helping solve real problems for real scientists working in oncology diagnostics and research.

In a moment I will hand over to Kimberly Holden, research associate from Labcorp, to talk us through their work exploring how automated size selection can be used to gain efficiency and improve quality in liquid biopsy.

Kimberly will introduce to us and explain the concept of circulating tumour DNA, and its importance in early detection and monitoring of cancer. She will then walk us through their study from methods to conclusions. Before this, I would like to give a short introduction to Ranger® Technology from Yourgene Health, which is the key technology used in the Kimberly’s study.

So what is Ranger® Technology, and why is it important in liquid biopsy? Kimberly will certainly take us deep in to this topic, but briefly Ranger® Technology offers industry-leading scalability and precision for the DNA size selection process, ensuring maximal enrichment every time. Core size selection functionality is complemented by the ability to perform fragment length analysis and fluorescence assays, so this is the perfect solution for next-generation sequencing (NGS) quality control applications.

Ranger® Technology has so far been deployed to power multiple solutions across our instrumentation capabilities, with applications from liquid biopsy to bacterial clonality assessment and beyond. So just briefly what are those instrumentation capabilities? The LightBench® is Yourgene’s cornerstone solution, and is already being used by laboratories across the DNA sequencing community. The NIMBUS Select, in partnership with Hamilton Robotics, is the solution of choice for our partners with higher throughput requirements, whilst the Yourgene® QS250 is available exclusively for use in Yourgene’s NIPT workflows.

At Yourgene we’re all about enabling scientific advances to positively impact human health, and that mission translates in to what we’re doing today with Ranger® Technology. We want to help laboratories in all fields and across multiple industries realise the benefits that come with optimising their NGS workflows through automated size selection, enrichment and quantification. We passionately believe in getting the message out there that if you’re working in NGS, this is a problem for your laboratory whether you know it or not! And we have the solution.

And ultimately our vision is to help your laboratory, whatever field you’re in whether it’s liquid biopsy or NIPT or bacterial genomics, accomplish more with less. And that applies in many ways – less capex equipment because our instruments are three-in-one solution. Less time, because our instruments offer true ‘walk away’ automation. Or less DNA, because ultimately you can achieve so much more if you’re only working with the DNA you care about and not wasting resource on sequencing off target fragments.

We have created this webinar with your learning in mind, and as such have introduced chapters and annotations to the recording to allow ease of navigation for a truly on demand experience. If you have any questions, our contact details are shown on the slide, and will also be available at the end of Kim’s talk. Thank you again to the team at Labcorp, and particularly Kimberly, for putting together this presentation for us. We hope you will find this useful, and thank you for your interest in this important work.

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